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Objective: To identify bioactive compound in pigeon pea leaves(Cajanus cajan) that inhibits Salmonella thypi(S. thypi).Methods: The leaf sample was powdered and macerated with methanol and fractioned by liquid-liquid extraction using ethyl acetate. The fraction was chromatographed and the isolates were identified for major component with liquid chromatography-mass spectrometry and the antibacterial activity was tested against S. thypi by Kirby-Bauer method.Results: Subtraction 1 from the ethyl acetate fraction formed a yellowish solid with m/z 272, identified as naringenin. The naringenin-rich fraction shows fairly well inhibitory toward S. thypi in comparison with chloramphenicol.Conclusions: Naringcnin shows antibacterial activity and can be developed to treat typhoid.
Objective: To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi). Methods: The leaf sample was powdered and macerated with methanol and fractioned by liquid-liquid extraction using ethyl acetate. The fraction was chromatographed and the isolates were identified for major component with liquid chromatography-mass spectrometry and the antibacterial activity was tested against S. thypi by Kirby-Bauer method. Results: Subtraction 1 from the ethyl acetate fraction formed a yellowish solid with m / z 272, identified as naringenin. The naringenin-rich fraction shows fairly well inhibitory toward S. thypi in comparison with chloramphenicol. Conclusions: Naringcnin shows antibacterial activity and can developed to treat typhoid.