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8只正常雄性大鼠血清总胆固醇(TCh)为0.5876±0.0874g/L((?)±SD),总甘油三酯(TG)0.7850±0.2417g/L,总磷脂(PL)1.2699±0.1022g/L,高密度脂蛋白胆固醇(HDL-Ch)0.4123±0.050 5g/L,HDL-PL 0.6190±0.0652g/L,HDL_3-Ch 0.1741±0.0290g/L,HDL_8-PL 0.4553±0.0807g/L,HDL_2-Ch 0.2381±0.0353g/L,HDL_2-PL 0.1638±0.0476g/L,极低密度脂蛋白胆固醇(VLDL-Ch)0.0618±0.0404g/L,VLDL-PL 0.1622±0.0869g/L,低密度脂蛋白胆固醇(LDL-Ch) 0.1136±0.0713g/L,和LDL-PL 0.4887±0.1230。门静脉注射1 ml纯化大鼠肝内皮细胞脂酶(HEL)1 h后血清TCh下降0.0425±0.0197 g/L(p<0.005),TG下降0.0964±0.0573g/L(p<0.005),PL下降0.1049±0.0516g/L(p<0.001),HDL-Ch下降0.0444±0.0289g/L(p<0.005),HDL-PL下降0.0675±0.0427g/L(p<0.005),HDL_3-Ch升高0.0055±0.0200g/L(p>0.5),HDL_3-PL升高0.0246±0.0482g/L,HDL_2-Ch下降0.0499±0.0429g/L(p<0.02),HDL_2-PL下降0.0918±0.0519g/L(p<0.002),VLDL-PL下降0.0136±0.0818g/L(p>0.5),VLDL-Ch下降0.0328±0.0403g/L(p<0.05),LDL-Ch升高0.0346±0.0262g/L(p<0.01),LDL-PL下降0.0239±0.0770g/L(p>0.02)。实验结果提示:(1)HDL-PL是HEL最适宜底物;(2)HEL是HDL_2分解代谢的酶,可
The serum total cholesterol (TCh) of 8 normal male rats was 0.5876 ± 0.0874 g / L (± SD), the total triglyceride (TG) was 0.7850 ± 0.2417 g / L and the total phospholipid (PL) was 1.2699 ± 0.1022 g / L, high density lipoprotein cholesterol (HDL-Ch) 0.4123 ± 0.050 5g / L, HDL-PL 0.6190 ± 0.0652g / L, HDL_3-Ch 0.1741 ± 0.0290g / L, HDL_8-PL 0.4553 ± 0.0807g / HDL_2-Ch 0.2381 ± 0.0353g / L, HDL_2-PL 0.1638 ± 0.0476g / L, VLDL-Ch 0.0618 ± 0.0404g / L, VLDL-PL 0.1622 ± 0.0869g / L, Lipoprotein cholesterol (LDL-Ch) 0.1136 ± 0.0713 g / L, and LDL-PL 0.4887 ± 0.1230. Serum TCh decreased by 0.0425 ± 0.0197 g / L (p <0.005), TG decreased by 0.0964 ± 0.0573 g / L (p <0.005), and PL decreased by 0.1049 (P <0.05) after portal vein injection of 1 ml purified rat liver lipase (HEL) HDL-Ch decreased 0.0444 ± 0.0289g / L (p <0.005), HDL-PL decreased 0.0675 ± 0.0427g / L (p <0.005), HDL_3-Ch increased 0.0055 ± 0.0200g / L (p> 0.5), HDL_3-PL increased 0.0246 ± 0.0482g / L, HDL_2-Ch decreased 0.0499 ± 0.0429g / L <0.002), 0.0136 ± 0.0818 g / L (p> 0.5), 0.0328 ± 0.0403 g / L (p <0.05) and 0.0346 ± 0.0262 g / L for VLDL- 0.01), LDL-PL decreased 0.0239 ± 0.0770g / L (p> 0.02). The experimental results suggest that: (1) HDL-PL is the most suitable substrate for HEL; (2) HEL is an HDL-2 catabolism enzyme