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目的:建立同时检测丹参多酚酸提取物中丹酚酸Y、迷迭香酸、紫草酸、丹酚酸B的HPLC-UV方法。方法:采用色谱柱Agilent Eclipse Plus C18(4.6 mm×250 mm,5μm);流动相为0.05%磷酸水-乙腈(78∶22),等度洗脱;流速0.7 m L·min-1;检测波长288 nm;柱温25℃。结果:迷迭香酸线性回归方程为Y=30.268X-12.638,R2=0.9997,线性范围4.00~40.00μg·m L-1,平均回收率101.05%(RSD 2.74%);紫草酸线性回归方程Y=17.554X-15.392,R2=0.9996,线性范围5.28~52.80μg·m L-1,平均回收率102.08%(RSD 3.19%);丹酚酸B线性回归方程Y=16.535X-204.7,R2=0.9997,线性范围为54.40~544.00μg·m L-1,平均回收率101.76%(RSD 2.16%);丹酚酸Y线性回归方程为Y=16.53X-58.335,R2=0.9997,线性范围为11.44~114.40μg·m L-1,平均回收率99.65%(RSD 3.90%)。结论:该方法操作简单、重复性好,可用于丹参多酚酸提取物中迷迭香酸、紫草酸、丹酚酸B和丹酚酸Y的含量同时测定。
OBJECTIVE: To establish a HPLC-UV method for the simultaneous determination of salvianolic acid Y, rosmarinic acid, lithospermic acid and salvianolic acid B in Salvia miltiorrhiza polyphenol extract. Methods: The mobile phase consisted of 0.05% phosphoric acid water-acetonitrile (78:22) with isocratic elution at a flow rate of 0.7 m L · min-1 using a Agilent Eclipse Plus C18 column (4.6 mm × 250 mm, 5 μm) 288 nm; column temperature 25 ℃. Results: The linear regression equation of rosmarinic acid was Y = 30.268X-12.638, R2 = 0.9997, the linear range was 4.00-40.00μg · m L-1, the average recovery was 101.05% (RSD 2.74%). = 17.554X-15.392, R2 = 0.9996, the linear range of 5.28 ~ 52.80μg · m L-1, the average recovery rate of 102.08% (RSD 3.19%); salvianolic acid B linear regression equation Y = 16.535X-204.7, R2 = 0.9997 , The linear range was 54.40 ~ 544.00μg · m L-1, the average recovery was 101.76% (RSD 2.16%). The linear regression equation of salvianolic acid was Y = 16.53X-58.335, R2 = 0.9997 with linear range of 11.44-114.40 μg · m L-1, the average recovery was 99.65% (RSD 3.90%). Conclusion: The method is simple and reproducible. It can be used for the simultaneous determination of rosmarinic acid, lithosperminic acid, salvianolic acid B and salvianolic acid Y in Salvia miltiorrhiza polyphenol extracts.