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我们于1979年开始了杉木组培育苗试验,已育出小苗,移栽到苗圃,生长正常。具体做法简介于下: 一、材料选用杉木优树1年生粗壮枝条,在接种室内切取带顶梢部分约3厘米长的枝条,经消毒后,切取1—2毫米的顶梢或顶梢下的茎段1—2块进行培养。二、方法1.培养基用MS或马铃薯简化培养基(马铃薯汁10—15%、蔗糖3—5%、琼脂0.7%),另外附加2,4-D 2—5毫克/升,糠基氨基嘌呤1—2ppm或椰汁10%。接种后全暗培养一星期后,即转光暗交替培养。白天光照8—10小时,用2支40瓦的日光灯作辅助光源,
We started the experiment of fir seedling cultivation in 1979, and we have raised seedlings and transplanted them to the nursery to grow normally. Specific approach Briefing on the following: First, the material selection fir tree 1-year-old robust branches, cut in the inoculation with the top part of about 3 cm long branches, after disinfection, cut 1-2 mm top or top of the shoot 1-2 stems were cultured. 2. Method 1. Culture medium MS or potato simplified medium (potato juice 10-15%, sucrose 3-5%, agar 0.7%), supplemented with 2,4-D 2-5 mg / l, furfurylamino Purine 1-2ppm or coconut milk 10%. After inoculation all dark culture a week later, the light dark alternate culture. Daylight 8-10 hours, with two 40-watt fluorescent lamp as a secondary light source,