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目的:建立稳定戊巴比妥钠致大鼠急性心功能不全模型并比较不同种类抗心功能不全药物的作用差异,籍此探讨该动物模型的适用性。方法:将雄性Wistar大鼠分为模型组、多巴酚丁胺组、去乙酰毛花苷组及黄芪注射液组,采用戊巴比妥钠股静脉给药方法建立大鼠急性心功能不全模型,即时用多巴酚丁胺(100μg·kg-1)、去乙酰毛花苷(0.1 mg·kg-1)或黄芪注射液(4 mL·kg-1)静脉给药干预,全过程中使用四道生理仪监测心电图、左心室内压等指标的变化,观察药物治疗效果1～2 h。结果:使用戊巴比妥钠建立的稳定大鼠急性心功能不全模型,左室内压峰值(LVSP)及左室压最大上升速率(dp.dt max-1)等各项指标降至初始值60%～70%并持续90 min以上。给予多巴酚丁胺治疗后2 min左右LVSP及dp.dtmax-1均明显回升至初始值90%以上(P<0.05),给予黄芪注射液治疗后20 min左右LVSP及dp.dtmax-1也明显回升至初始值90%左右(P<0.05),但使用去乙酰毛花苷注射液治疗后未观察到指标明显改善现象。结论:使用戊巴比妥钠可建立较为稳定大鼠急性心功能不全模型,此模型比较适用于多巴酚丁胺类药物及黄芪注射液的药理及药效研究,而不太适用于去乙酰毛花苷类药物的药效及深入机制研究。
OBJECTIVE: To establish a stable rat model of acute cardiac insufficiency induced by pentobarbital sodium and to compare the effects of different types of anti-heart insufficiency drugs, so as to explore the applicability of the animal model. Methods: Male Wistar rats were divided into model group, dobutamine group, stilbene group and astragalus injection group. Rats were induced by acute femoral vein with pentobarbital sodium , Immediate use of dobutamine (100μg · kg-1), stilbene (0.1 mg · kg-1) or Astragalus injection (4 mL · kg-1) intravenous intervention, the whole process using Four physiological monitoring ECG, left ventricular pressure and other indicators of change, observe the effect of drug treatment 1 ~ 2 h. Results: The steady-state rat model of acute cardiac insufficiency established by pentobarbital sodium reduced to the initial values of 60 (dp.dt max-1) and other indicators of left ventricular pressure peak (LVSP) and maximum left ventricular pressure % ~ 70% for more than 90 min. LVSP and dp.dtmax-1 significantly increased to more than 90% of the initial values at 2 min after dobutamine treatment (P <0.05), and LVSP and dp.dtmax-1 at 20 min after treatment with Astragalus injection Significantly increased to about 90% of the initial value (P <0.05), but no significant improvement was observed after treatment with strythin injection. Conclusion: A stable rat model of acute cardiac insufficiency can be established by pentobarbital sodium. This model is more suitable for pharmacological and pharmacodynamic studies of dobutamine and astragalus injection, but not suitable for deacetylation Study on the Pharmacodynamics and Deep Mechanism of Gliacynthus.