论文部分内容阅读
目的:比较量子点免疫荧光和传统免疫组化技术在检测人前列腺组织中前列腺特异性抗原(PSA)表达的效果。方法:用两种方法分别标记人前列腺组织中的PSA,对比两种方法的标记效果,并在实验后1个月内观察量子点免疫萤光标记的稳定性,于实验后当天及20d和30d对荧光强度和密度分成4个等级进行比较。结果:两种方法分别标记人前列腺组织中PSA的效果具有一致性(K=0.910,P<0.001),在前列腺癌组织中PSA的表达均随着肿瘤分级(量子点免疫荧光,r=-0.802,P<0.001;免疫组化,r=-0.749,P<0.001)和分期(量子点免疫荧光,r=-0.745,P<0.001;免疫组化,r=-0.659,P<0.001)的增高而降低;另外,在PSA阳性表达的组织中,量子点荧光的强度及密度在实验10d后未发生明显变化(P=0.157),在实验30d后仍有97.2%清晰可辨。结论:量子点免疫荧光技术是一种有效、稳定的检测技术,具有广阔的应用前景。
OBJECTIVE: To compare the effect of quantum dot immunofluorescence and traditional immunohistochemistry in the detection of prostate-specific antigen (PSA) expression in human prostate tissue. Methods: The PSA in human prostate tissue was labeled by two methods respectively. The stability of immunofluorescent labeling of quantum dots was observed within 1 month after the experiment. On the day after the experiment and on the days of 20d and 30d Fluorescence intensity and density were divided into 4 levels. Results: The results of two methods were consistent (P = 0.001). The expression of PSA in prostate cancer tissue was consistent with the tumor grade (QD, r = -0.802 , P <0.001; immunohistochemistry, r = -0.749, P <0.001) and staging (QD immunofluorescence, r = -0.745, P <0.001; immunohistochemistry, r = -0.659, P <0.001) In addition, in the PSA-positive tissues, the intensity and density of quantum dot fluorescence did not change significantly after 10 days (P = 0.157), and 97.2% were still clearly distinguishable 30 days after the experiment. Conclusion: Quantum Dot Immunofluorescence is an effective and stable detection technique with broad application prospects.