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①在质量浓度为５．０ｇ·Ｌ￣（－１）的氯化钠胁迫条件下，杨树外植体嫩茎的芽分化能力比嫩叶和根的强。嫩茎作为组培耐盐种质筛选的外植体是最适宜的。②在５．０ｇ·Ｌ￣（－１）盐胁迫条件下，无性系ＮＬ－８０１０５和ＮＬ－８０１０６的芽分化能力比无性系ＮＬ－８０３０３的强，以无性系ＮＬ－８０１０５为最强。③采用ＭＳ为基本培养基，附加６－ＢＡ０．４０ｍｇ·Ｌ－１、ＮＡＡ０．０２ｍｇ·Ｌ￣（－１）的浓度组合筛选耐盐再生小植株较为适宜。④经过逐渐提高盐浓度的分阶段筛选法，获得了能耐质量浓度为１０．０ｇ·Ｌ－１氯化钠的愈伤组织系，耐１０．０ｇ·Ｌ￣（－１）氟化钠和１０．０ｇ·Ｌ￣（－１）海水盐的无性系ＮＬ－８０１０５和ＮＬ－８０１０６不定芽若干以及耐７．５ｇ·Ｌ￣（－１）氯化钠的无性系ＮＬ－８０１０５再生小植株２１株和天性系ＮＬ－８０１０６再生小植株２株。
① Under the NaCl stress of 5.0 g · L -1, the shoot bud differentiation ability of poplar explants was stronger than that of young leaves and roots. It is the most suitable explants for the selection of explants for tissue culture salt-tolerant germplasms. ② Under 5.0g · L ~ (-1) salt stress, the budding ability of NL-80105 and NL-80106 was stronger than that of NL-80303. The clonal NL-80105 was the strongest. ③ It is more suitable to select the salt-tolerant regeneration plantlets by using MS as basic medium with the concentration of 6-BA0.40mg · L-1 and NAA0.02mg · L ~ (-1). ④ After gradually increasing the salt concentration of the phased screening method, obtained resistant to the concentration of 10.0g · L-1 sodium chloride callus lines, resistant to 10.0g · L ~ (-1) sodium fluoride and 10 .0g · L ~ (-1) seawater salt clones NL-80105 and NL-80106 adventitious buds and 7.5g · L ~ (-1) sodium chloride clonal NL-80105 regenerated plantlets 21 And nature line NL-80106 regenerate 2 plantlets.