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本文构建具有DNA聚合酶链式反应(PCR)和毛细管电泳(CE)分离检测功能的微流体芯片uC/OS-II嵌入式实时控制系统。本系统采用32位嵌入式微控制器ARM实现PCR扩增所需的闭环温度控制功能,毛细管电泳分离功能所需的高压电场自动调度功能。uC/OS-II嵌入式实时操作系统加强了系统的实时性。模糊免疫PID控制算法实现对温度的精确控制,其控制性能远优于常规PID控制器。试验结果表明本系统不但完全满足设计要求,而且与同功能的仪器平台相比,实时性强,不再需要昂贵的个人电脑进行控制运算和常用的DNA检测设备进行检测,实现了PCR-CE微流体芯片系统的低成本和小型化。
In this paper, a microfluidic chip uC / OS-II embedded real-time control system with DNA polymerase chain reaction (PCR) and capillary electrophoresis (CE) The system uses 32-bit embedded microcontroller ARM to achieve the required closed-loop PCR temperature control function, capillary electrophoresis separation function required for high-voltage electric field automatic scheduling function. uC / OS-II embedded real-time operating system to enhance the real-time system. Fuzzy immune PID control algorithm to achieve precise temperature control, its control performance is much better than the conventional PID controller. Experimental results show that the system not only fully meets the design requirements, but also real-time compared with the same functional instrument platform, eliminating the need for expensive personal computers for control computing and commonly used DNA testing equipment to detect, to achieve the PCR-CE micro Low cost and miniaturization of fluidic chip systems.