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目的:建立咽立爽口含滴丸的鉴别与含量测定方法。方法:采用TLC对甘草酸单铵盐进行定性鉴别;采用HPLC以羟基苯甲酸丁酯为内标物测定甘草酸单铵盐含量,流动相乙腈-0.01 mol·L-1磷酸溶液(38∶62),流速1.0 mL·min-1,检测波长250 nm,柱温25℃。结果:该方法专属性好、准确,甘草酸单铵盐在0.212~3.71 mg具有良好线性关系(r=0.999 9),平均回收率为101.07%。结论:该法简便,快速,准确,可用于产品的质量控制。
Objective: To establish the method of identification and content determination of pharynx and refreshing drop pills. Methods: Qualitative identification of glycyrrhizic acid monoammonium salt was carried out by TLC. Content of monoammonium glycyrrhizinate was determined by HPLC with butyl hydroxybenzoate as internal standard. The mobile phase was acetonitrile-0.01 mol·L-1 phosphoric acid solution (38:62 ), Flow rate 1.0 mL · min-1, detection wavelength 250 nm, column temperature 25 ℃. Results: The method was of good specificity and accuracy. The linearity of glycyrrhizin monoammonium salt was 0.212 ~ 3.71 mg (r = 0.999 9) with the average recovery of 101.07%. Conclusion: The method is simple, rapid and accurate and can be used for product quality control.