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利用水提醇沉法对桦褶孔菌Lenzites betulina粗多糖HZKJc进行提取,随后经Sevage法和酶法联合脱蛋白,再经DEAE‐Sephadex A‐25,G‐100柱层析纯化后得到多糖纯品HZKJv。通过高效液相色谱(HPLC)测定上述纯化多糖的纯度和相对分子量分布;经纸层析(PC)、气相色谱(GC)、红外光谱(IR)进行单糖组成分析;并研究其清除自由基活性。分离纯化后得到的HZKJv为纯多糖,相对分子质量约为11 687。当HZKJv溶液浓度为3.0mg/m L时,其DPPH自由基清除率可达82%;浓度为2.0mg/m L时,对?OH自由基的清除率可达85%。表明HZKJv对?OH与DPPH自由基存在很好的清除作用。
The water extract alcohol precipitation method was used to extract HZKJc from Lenzites betulina crude polysaccharide, and then deproteinized by Sevage method and enzymatic method, and purified by DEAE-Sephadex A-25 and G-100 column chromatography to obtain pure polysaccharide Product HZKJv. The purity and relative molecular weight distribution of the purified polysaccharides were determined by high performance liquid chromatography (HPLC). The composition of monosaccharides was analyzed by paper chromatography (PC), gas chromatography (GC) and infrared spectroscopy (IR) active. HZKJv isolated and purified to pure polysaccharides, the molecular weight of about 11,687. When the concentration of HZKJv solution was 3.0mg / m L, the DPPH radical scavenging rate could reach 82%. When the concentration of HZKJv solution was 2.0mg / m L, the scavenging rate of OH radical was up to 85%. It shows that HZKJv has a good scavenging effect on? OH and DPPH free radicals.