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目的建立腰舒胶囊的定性鉴别和含量测定方法。方法用薄层色谱(TLC)法定性鉴别制剂中川牛膝、当归、狗脊、党参、槲寄生、丹参。用高效液相色谱(HPLC)法测定制剂中丹参酮ⅡA的含量,以氨基键合硅胶为填充剂,以乙腈∶水(8∶2)为流动相,检测波长为250 nm。结果薄层色谱斑点清晰,分离度好,阴性对照无干扰;丹参酮ⅡA进样量在1.0~10.0μg/mL范围内与其峰面积积分值线性关系良好,平均回收率为92.80%。结论所建立的定性、定量分析方法可用于颈舒胶囊的质量控制。
Objective To establish the qualitative identification and determination of waist Shu capsules. Methods Thin layer chromatography (TLC) was used to identify the preparations of Achyranthes bidentata, Angelica sinensis, Dorsal vertebra, Codonopsis, Mistletoe and Salvia miltiorrhiza. The content of tanshinone ⅡA in the preparation was determined by high performance liquid chromatography (HPLC). Amino-bonded silica gel was used as filler and acetonitrile / water (8: 2) was used as mobile phase. The detection wavelength was 250 nm. Results The TLC spots were clear and the resolution was good. There was no interference with the negative control. The linear range of the tanshinone ⅡA injection was 1.0 ~ 10.0 μg / mL, with an average recovery of 92.80%. Conclusion The established qualitative and quantitative analysis methods can be used for the quality control of Jingshu capsule.