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目的:研究SLP-2(stomatin like protein2)基因在结肠癌及正常结肠黏膜中的表达情况.方法:应用RT-PCR法及免疫组织化学法分别检测40例和50例结肠癌及其癌旁正常结肠黏膜中SLP-2mRNA和蛋白的表达,并结合免疫组织化学结果及结肠癌患者的临床病理资料进行分析.结果:RT-PCR结果显示,结肠癌及配对癌旁正常组织中SLP-2mRNA的表达水平分别为1.31±0.28和0.74±0.16,前者较后者明显升高(P<0.05).免疫组织化学结果显示,SLP-2蛋白在结肠癌及配对癌旁正常组织中的高表达率分别为70.0%(35/50)和22%(11/50),两者差异有统计学意义(P<0.05).且SLP-2蛋白的表达与有无淋巴结转移及TNM分期相关(均P<0.05).结论:SLP-2基因在结肠癌组织中高表达,可能参与结肠癌的发生发展和转移.
Objective: To study the expression of SLP-2 (stomatin like protein2) gene in colon cancer and normal colorectal mucosa.Methods: RT-PCR and immunohistochemistry were used to detect the expression of 40 and 50 normal colorectal cancer The expression of SLP-2mRNA and protein in colonic mucosa were analyzed by immunohistochemistry and clinicopathological data.Results: The expression of SLP-2mRNA in colon cancer and adjacent normal tissues (P <0.05). The results of immunohistochemistry showed that the high expression rates of SLP-2 protein in normal tissues of colorectal cancer and paired cancer tissues were 1.31 ± 0.28 and 0.74 ± 0.16, respectively 70.0% (35/50) and 22% (11/50), respectively (P <0.05), and the expression of SLP-2 protein was correlated with lymph node metastasis and TNM staging ) Conclusion: SLP-2 gene is highly expressed in colon cancer tissues, which may be involved in the development and metastasis of colon cancer.