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目的:观察急性冠状动脉综合征(ACS)患者外周血清可溶性CD40配体(sCD40L)的水平、外周血单个核细胞(PBMCs)中核因子kappa B(NF-κB)的活性及两者与基质金属蛋白酶-9(MMP-9)之间的关系,探讨ACS预测和治疗的新思路。方法:采用酶联免疫吸附法测定所有入选对象外周血清sCD40L、MMP-9的浓度及PBMCs中NF-κB的活性。结果:ACS组外周血清sCD40L的浓度及PBMCs中NF-κB的活性均高于稳定型心绞痛组和正常对照组,均差异有统计学意义(P<0.05);ACS组血清sCD40L水平、PBMCs中NF-κB的活性与MMP-9呈显著正相关(P<0.01)。结论:ACS患者血清sCD40L水平及PBMCs中NF-κB的活性升高,可能是动脉粥样硬化斑块不稳定的标志之一;CD40L、NF-κB可能通过促使MMP-9的表达增加而促使ACS的发生。
OBJECTIVE: To investigate the levels of soluble CD40 ligand (sCD40L) in peripheral blood and activity of nuclear factor kappa B (NF-κB) in peripheral blood mononuclear cells (PBMCs) in patients with acute coronary syndrome (ACS) and their relationship with matrix metalloproteinases -9 (MMP-9), to explore the new idea of ACS prediction and treatment. Methods: The concentrations of sCD40L, MMP-9 in peripheral blood serum and the activity of NF-κB in PBMCs were determined by enzyme-linked immunosorbent assay. Results: The serum concentration of sCD40L and the activity of NF-κB in PBMCs in ACS group were significantly higher than those in stable angina group and normal control group (all P <0.05). Serum levels of sCD40L and NF The activity of -κB was positively correlated with MMP-9 (P <0.01). Conclusion: Serum levels of sCD40L and NF-κB in PBMCs may be one of the markers of atherosclerotic plaque instability in ACS patients. CD40L and NF-κB may promote ACS by increasing the expression of MMP-9 happened.