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目的:对我省历年分离的不同地区和来源EHEC O157:H7菌株之间的同源性进行分析。方法:用限制性内切酶XbaI,对分离菌株进行PFGE分型,并使用BioNumerics Version 4.0软件(Dice系数和UPGMA法)进行聚类分析。结果:74株O157:H7分离株可分为39个型,同一年份同一地区不同来源菌株之间有相同的XbaI酶切带型,同一年份不同地区分离菌株之间有相同酶切带型,不同年份部分菌株之间酶切带型不可区分。结论:我省宿主动物携带多个O157:H7克隆,来自同一个克隆的O157:H7已在较广泛的区域内传播,某些在1999年暴发流行的克隆长期稳定存在于我省宿主动物中,并传播给人类。
OBJECTIVE: To analyze the homology between EHEC O157: H7 strains in different regions and sources isolated in our province over the years. Methods: The isolated strains were subjected to PFGE typing using restriction endonuclease XbaI and clustering analysis was performed using BioNumerics Version 4.0 software (Dice Coefficient and UPGMA method). Results: The results showed that 74 strains of O157: H7 could be divided into 39 types. In the same year, the same strain of XbaI was found between different strains in the same year. In the same year, strains isolated from different regions had the same digestion pattern Some strains of the year between the digestion type can not be distinguished. Conclusion: The host animals in our province carry multiple O157: H7 clones. O157: H7 from the same clone has spread in a wide range of areas. Some of the clones that broke out in 1999 were long-term stable in host animals of our province, And spread to humans.