论文部分内容阅读
目的 :观察大鼠 5 /6肾切除模型中间质微血管病变的特点及药物缬沙坦和雷米普利对其的干预作用。 方法 :以SD大鼠 5 /6肾切除建立肾间质纤维化 (RIF)动物模型 ,设正常组 (n =6 ) ,假手术组 (n =6 ) ,手术组(n =7) ,氨氯地平组 (n =6 ) ,缬沙坦组 (n =10 ) ,雷米普利组 (n =10 )。术后定时测定 2 4h尿蛋白排泄量及血压。 12周后取材 ,留取血样 ,2 4h尿样及肾组织。常规病理检查判断RIF及肾小管间质损伤程度 ;应用免疫组化方法显示肾脏毛细血管。 结果 :与正常组和假手术组相比 ,手术组RIF明显 ,间质微血管病变严重 ,肾小管周围毛细血管(PTC)密度明显减少 (36 3 2 9± 70 .99vs 798.17± 6 6 .5 3,776 .10± 5 1.2 9,P <0 0 1)。而缬沙坦和雷米普利能延缓RIF及间质微血管病变 ,并改善肾功能 ,与手术组相比 ,两组PTC密度均明显改善 (45 5 5 0± 6 2 .98,P <0 .0 5和 4 6 5 6 0±5 7 38,P <0 0 1)。而氨氯地平血压控制组与手术组PTC密度无差异。 结论 :间质微血管病变不仅是RIF的特征性病理改变 ,而且在RIF及肾功能减退中可能起重要作用。缬沙坦和雷米普利延缓RIF和对肾功能的保护作用与间质微血管病变的改善有关 ,其机制可能与血管紧张素Ⅱ相关联。
OBJECTIVE: To observe the characteristics of interstitial microangiopathy in rat model of 5/6 nephrectomy and the intervention of valsartan and ramipril. Methods: The animal model of renal interstitial fibrosis (RIF) was established by 5/6 nephrectomy of SD rats. The normal group (n = 6), sham operation group (n = 6), operation group Chlorpheniramine (n = 6), valsartan (n = 10) and ramipril (n = 10). Twenty-four hours after operation, urinary protein excretion and blood pressure were measured. Draw after 12 weeks, blood samples were taken, 24 h urine samples and kidney tissue. Routine pathological examination to determine the extent of RIF and tubulointerstitial injury; immunohistochemical methods of renal capillaries. Results: Compared with the normal group and the sham operation group, the RIF of the operation group was obvious, the interstitial microvascular lesions were serious and the density of the peritubular capillary (PTC) was significantly decreased (36 3 2 9 ± 70 .99 vs 798.17 ± 6 6 .5 3,776 .10 ± 5 1.2 9, P <0 0 1). However, valsartan and ramipril delayed the RIF and interstitial microangiopathy, and improved renal function. Compared with the surgery group, the PTC density was significantly improved in both groups (45 55 ± 6 2 .98, P 0 .0 5 and 4 6 5 6 0 ± 5 7 38, P <0 0 1). The amlodipine blood pressure control group and surgery group no difference in PTC density. Conclusion: Interstitial microangiopathy is not only a characteristic pathological change of RIF, but also plays an important role in RIF and renal dysfunction. Valsartan and Ramipril delay RIF and renal function and the protective effect of interstitial microangiopathy improvement, the mechanism may be associated with angiotensin Ⅱ.