MorphologiCal and Cytogenetic Analysis on the Advanced Generations of Generic Hybrids between Brassi

• 作 者: Zhigang ZHAO Dezhi DU Zaiyun L
• 机 构: National Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm of Qi
• 出 处: 农业科学与技术：英文版
• 发表日期: 2004年期
• 关键词: Brassica napus Orychophragmus violaceus Sexual hybrids Hypoploid
• 基金项目: the Special Fund for Preliminary Study of the National Basic Research Program of China

Objective This study aimed to reveal the genetic changes of advanced generation hybrids between Brassica napus and Orychophragmus violaceus. [Method] The morphological characteristics such as the plant shape, branching sites, leaf shape, leaf color, primary branches and secondary branches, as well as the cytolog- ical characteristics of the advanced generation hybrids （Fs-F10） between B. napus and O. violaceus were observed. [Result] The morphology analysis revealed that the hybrid progeny was more like B. rapa in leaf shape, leaf color, plant shape and ear- ly flowering phenotype, whereas more like B. napus in number of secondary branch- es, silique length and 1 000-seed weight. Analysis on the cytogenetics characteristics showed that these advanced inbred progenies were hypoploids with less than 38 chromosomes; moreover, all the chromosomes from O. violaceus had been lost. Chromosome pairings at meiotic diakinesis of hybrids between the advanced inbred progenies and B. rapa revealed that chromosomes lost in hypoploids possibly be- longed to the C genome of B. oleracea. With generations developing, chromosomes number of plants from two populations gradually increased and developed into the number of B. napus （2n=38）. [Conclusion] This study will provide reference to reveal the source of chromosome lost in hypoploids and the morphological change of hybrids.