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We report the structural and electrical transport properties of Fe1-xCuxSe(x = 0,0.02,0.05,0.10)single crystals grown by a chemical vapor transport method.Substituting Cu for Fe suppresses both the nematicity and superconductivity of FeSe single crystal,and provokes a metal-insulator transition.Our Hall measurements show that the Cu substitution also changes an electron dominance at low temperature of un-doped FeSe to a hole dominance of Cu-doped Fe1-xCuxSe at x = 0.02 and 0.1,and reduces the sign-change temperature(TR)of the Hall coefficient(RH).