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目的：在大鼠主动脉球囊剥脱模型上观察血浆和主动脉组织Ｃ型利钠利尿肽（Ｃｔｙｐｅ ｎａｔｒｉｕｒｅｔｉｃ ｐｅｐｔｉｄｅ，ＣＮＰ）的质量浓度和ｍＲＮＡ 表达的动态变化及外源性ＣＮＰ对大鼠球囊损伤后内膜生成的影响，以探讨ＣＮＰ在再狭窄中的作用。方法：放射免疫法测定ＣＮＰ质量浓度，ＲＴＰＣＲ 测定ＣＮＰｍＲＮＡ 的表达。结果：发现大鼠球囊损伤后血浆ＣＮＰ的质量浓度升高， 内膜剥脱后第３ 天、１０ 天、２１ 天，血浆ＣＮＰ 水平分别增高８０ ．７％ （ Ｐ＜ ０．０１） ，４３．５ ％ （Ｐ＜ ０．０５） 和２７ ．５％ （Ｐ＜ ０．０５），而主动脉组织ＣＮＰ含量在损伤后第３ 天下降４６ ．６％ （Ｐ＜ ０．０５） ，第１０ 天，２１ 天和２８ 天ＣＮＰ含量分别增加２ ．８ 倍（ Ｐ＜ ０ ．０１） 、１．６ 倍（ Ｐ＜ ０ ．０５） 和０ ．８２ 倍（Ｐ＜ ０．０５）。在血管损伤３ 天时ＣＮＰｍＲＮＡ 的表达减弱，而第１０ 天和第２１ 天ＣＮＰｍＲＮＡ 的表达明显增强。外源性ＣＮＰ显著抑制球囊损伤后第７ 天和２１ 天新生内膜的形成，内膜／中膜比值分别降低２３％ （Ｐ＜ ０．０５）和２０ ％ （Ｐ＜０ ．０５）。结论：ＣＮＰ参与血管球囊损伤后的修复过程，可能是机体?
OBJECTIVE: To observe the dynamic changes of C-type natriuretic peptide (C-type natriuretic peptide, CNP) mRNA and mRNA expression in rat aortic balloon balloon dissection and the effect of exogenous CNP on Intima formation after balloon injury in rats to explore the role of CNP in restenosis. Methods: CNP concentration was determined by radioimmunoassay and CNP mRNA expression was determined by RT-PCR. Results: The plasma concentration of CNP was increased after balloon injury in rats. The plasma CNP levels increased by 80 on the 3rd, 10th and 21st days after endothelium stripping. 7% (P <0.01), 43.5% (P <0.05) and 27 respectively. 5% (P <0.05), while CNP content in aortic tissue decreased 46 days after injury.3. 6% (P <0.05). CNP contents increased by 2 on the 10th, 21st and 28th days, respectively. 8-fold (P <0 .01), 1.6-fold (P <0 .05) and 0. 82-fold (P <0.05). The expression of CNP mRNA decreased 3 days after vascular injury, while CNP mRNA expression increased significantly on the 10th and 21st days. Exogenous CNP significantly inhibited neointima formation on day 7 and 21 after balloon injury, with intima / media ratios decreased by 23% (P <0.05) and 20% (P <0.05), respectively. Conclusion: CNP involved in the repair process of vascular balloon injury may be the body?