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以白木通的带芽茎段为外植体,研究植物生长调节剂对其离体培养及植株再生的影响,建立了木通离体快繁技术体系。结果表明:带芽茎段的较佳消毒灭菌方法为75%的酒精浸泡30 s,再用2%的次氯酸钠溶液浸泡15 min;腋芽诱导的最适宜培养基为WPM+1.0 mg·L-1 6-BA+0.5 mg·L-1 IAA+2.0 mg·L-1 GA3,诱导率可达81.27%;适合腋芽增殖的培养基为WPM+3.0mg·L-1 6-BA+0.1 mg·L-1 IBA,增殖系数可达4.26;最佳生根培养基为1/2MS+1.0 mg·L-1 IBA+0.5 mg·L-1 NAA+1.0 mg·L-1 GA3,生根率为82.18%。炼苗移栽到泥炭土:珍珠岩:蛭石=2:1:1的基质中,成活率达76.0%以上,该结果为木通的快繁提供了一条新途径。
In order to study the effect of plant growth regulators on the in vitro culture and plant regeneration, the stems with buds of Baimu were used as explants to establish a rapid propagation system of M. canadensis. The results showed that the optimal method of sterilization with bud stem segments was immersed in 75% alcohol for 30 s and soaked in 2% sodium hypochlorite solution for 15 min. The optimum medium for axillary bud induction was WPM + 1.0 mg · L -1 6-BA + 0.5 mg · L-1 IAA + 2.0 mg · L-1 GA3, the induction rate was 81.27%. The medium suitable for axillary bud proliferation was WPM + 3.0 mg · L-1 6-BA + 0.1 mg · L -1 IBA with a multiplication coefficient of 4.26. The best rooting medium was 1 / 2MS + 1.0 mg · L-1 IBA + 0.5 mg · L-1 NAA + 1.0 mg · L-1 GA3 with the rooting rate of 82.18%. The results showed that the survival rate of peat seedlings transplanted into peat soil: perlite: vermiculite = 2: 1: 1 was more than 76.0%, which provided a new way for the rapid propagation of wood.