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探讨3’—甲基—4—二甲氨基偶氮苯（3’－Me－DAB）诱发大鼠肝癌过程中肝脏磷脂代谢的变化。用3’－Me－DAB诱发Wistar大鼠肝癌，同时辅以低胆碱饮食，TLC分离磷脂组分，Rouser法测定第6、9、12、16、20周肝脏中总磷脂及各磷脂组分含量。结果：发现诱癌组总磷脂和磷脂酸胆碱（PC）的含量不断下降，且两者变化一致；鞘磷脂（SM）在3’—Me—DAB加普通饮食组（A组）诱癌早期即迅速增高，超过对照组约1倍，并一直维持至肝癌形成，而在3’—Me—DAB加低胆碱饮食组（B组）虽然早期也升高，但当第20周肿瘤发生转移时降至对照组以下；溶血磷脂酰胆碱（LPC）仅在B组晚期出现下降。不含胆碱基因的磷脂组分：磷脂酰乙醇胺（PE）、磷脂酰丝氨酸（PS）和磷脂酰肌醇（PI）在3’—Me—DAB诱癌晚期均降低，B组尤为明显。缩醛磷脂的含量在整个诱癌过程中未见明显变化。结论：3’－Me－DAB诱发大鼠肝癌过程中主要影响PC和SM的代谢，表现为PC降低，SM增高。低胆碱饮食能缯强3’—Me—DAB对大鼠肝脏磷脂代谢的影响。
To investigate the changes of hepatic phospholipid metabolism during liver cancer induced by 3’-methyl-4-dimethylaminoazobenzene (3’-Me-DAB) in rats. Wistar rat hepatoma induced by 3’-Me-DAB was supplemented with low choline diet. TLC was used to separate phospholipids. The total phospholipids and phospholipids in liver were determined at 6, 9, 12, 16 and 20 weeks by Rouuser method. content. RESULTS: The total phospholipids and phosphatidylcholine (PC) levels in the cancer-causing group decreased continuously, and the changes were consistent; Sphingomyelin (SM) was found in the early stage of cancer induction in the 3’-Me-DAB plus diet group (group A). That is, rapid increase, about 1 times higher than the control group, and maintained until the formation of liver cancer, while in the 3’-Me-DAB plus low choline diet group (B group) although early but also increased, but when the 20th week tumor metastasis At the same time, it dropped below the control group; Lysophosphatidylcholine (LPC) only decreased in the late stage B group. Phosphatidylcholine-free phospholipids: Phosphatidylethanolamine (PE), phosphatidylserine (PS), and phosphatidylinositol (PI) decreased in the late stage of 3’-Me-DAB induced cancer, especially in group B. The plasmalogen content did not change significantly throughout the cancer induction process. CONCLUSION: The metabolism of PC and SM is mainly affected by 3’-Me-DAB-induced liver cancer in rats, which is characterized by decreased PC and increased SM. Low choline diet can reluctantly influence 3’-Me-DAB on liver phospholipid metabolism in rats.