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目的检测脆性组氨酸三联体(FHIT)基因在胃癌(GC)、不典型增生(DP)和肠上皮化生(IM)组织中的杂合性丢失(loss of heterozygosity,LOH),研究LOH在GC发生中的作用。方法采用PCR方法检测GC、DP和IM组织和正常对照胃黏膜组织中FHIT基因多态性位点D3S1234和D3S1300中的LOH。结果 GC组、DP组和IM组在D3S1234位点的LOH发生率分别为33.0%,29.0%和11.0%;在D3S1300位点的LOH发生率分别为34.0%,33.0%和8.0%。GC组和DP组在D3S1234和D3S1300位点的LOH发生率显著高于IM组,GC组与DP组间差异无统计学意义。结论 FHIT基因的LOH可能是GC形成过程中的早期事件,能用于GC高危人群筛查和早期诊断的研究。
Objective To detect the loss of heterozygosity (LOH) of fragile histidine triad (FHIT) gene in gastric cancer (GC), atypical hyperplasia (DP) and intestinal metaplasia (IM) tissues, and to study the role of LOH in The role of GC in the occurrence. Methods The LOH of FHIT gene polymorphisms D3S1234 and D3S1300 in gastric mucosa tissues of GC, DP and IM tissues and normal controls were detected by PCR. Results The incidences of LOH at the D3S1234 locus in GC group, DP group and IM group were 33.0%, 29.0% and 11.0%, respectively. The incidence of LOH at D3S1300 locus was 34.0%, 33.0% and 8.0%, respectively. The incidences of LOH in GC group and DP group at D3S1234 and D3S1300 were significantly higher than those in IM group. There was no significant difference between GC group and DP group. Conclusions The LOH of FHIT gene may be an early event in the GC formation process and can be used for screening and early diagnosis of high-risk GC populations.