论文部分内容阅读
目的建立SD大鼠腹腔注射链脲霉素(STZ)致妊娠糖尿病的实验动物模型,为研究妊娠糖尿病对胎儿发育影响提供实验基础。方法将54只SD孕鼠随机分为STZ注射组(30只)和柠檬酸缓冲液注射对照组(24只),两组又按取材时孕龄不同随机分为第13、16天和19天3个亚组。对STZ注射组孕鼠,给予新配2%STZ溶液,按STZ40mg/kg,一次性腹腔注射,3d后空腹尾静脉采血监测血糖、尿糖和体重。对照组用同样方法注射等量的柠檬酸钠缓冲液。孕鼠于孕龄13、16、19d随机分批剖宫,观察完全流产、吸收胎、死胎及存活胎数等情况,检查子鼠的外部形态,测量子鼠体重。子鼠心脏切片经常规HE染色后,进行病理分析。结果STZ注射组孕鼠用药后出现“三多一少”现象,3d后血糖水平明显高于对照组(14.65±1.12)mmol/L比(3.06±0.39)mmol/L,t=48.29,P<0.01,尿糖均阳性,体重明显低于对照组(260.7±14.0)g比(273.7±16.7)g,t=3.11,P<0.01;13、16d和19d流产率均高于对照组,χ2分别为4.37、7.51和11.05,P<0.05;子鼠液化吸收胎、死胎、畸心胎例数较相应对照组高,χ2=77.21,P<0.01。STZ注射组子鼠心脏发育异常数较对照组多,F=196,P<0.01。结论该动物实验模型与人类妊娠糖尿病(GDM)拟合较好,且操作简便,重复性高,是研究妊娠糖尿病致胎儿发育异常较理想的实验模型。
Objective To establish an experimental animal model of gestosis caused by intraperitoneal injection of streptozotocin (STZ) in SD rats, and provide an experimental basis for studying the effects of gestational diabetes on fetal development. Methods Fifty-four SD pregnant rats were randomly divided into STZ injection group (n = 30) and citrate buffer injection control group (n = 24). The two groups were randomly divided into 13th, 16th and 19th day 3 subgroups. The pregnant rats in the STZ injection group were given 2% STZ solution, and the blood glucose, urine sugar and body weight were monitored by STZ40mg / kg, single intraperitoneal injection, 3d fasting tail vein blood sampling. The control group was injected with the same amount of sodium citrate buffer in the same manner. The pregnant rats were randomly divided into cesarean section at gestational age of 13, 16 and 19 days to observe the complete abortion, aborted fetus, stillbirth and the number of surviving fetuses. The external morphology of the offspring was examined and the weight of the offspring was measured. Rat heart sections were routine HE staining, pathological analysis. Results The blood glucose level of STZ injection group was significantly higher than that of control group (3.06 ± 0.39 mmol / L, t = 48.29, P < (P <0.01). The urine glucose was positive and the body weight was significantly lower than that of the control group (260.7 ± 14.0 g vs 273.7 ± 16.7 g, t = 3.11, P <0.01) χ2 = 4.37, 7.51, and 11.05 respectively, P <0.05. The number of liquefaction-absorbed fetus, stillbirth and malformed fetus in the offspring was higher than that of the corresponding control group, χ2 = 77.21, P <0.01. STZ injection group rats heart abnormalities than the control group, F = 196, P <0.01. Conclusion The experimental animal model of GDM is better than human gestational diabetes mellitus (GDM) and has the advantages of simple operation and high repeatability. It is an ideal experimental model for studying abnormal fetal development caused by gestational diabetes mellitus.