Prokaryotic Expression and Identification of the WbkC Gene of Brucella mefitensis

• 作 者: WANGXue li BURi e YANGMing xia
• 机 构: AnimalScienceandTechnologyCollege,LifeScienceCollege
• 出 处: 动物与饲料科学：英文版
• 发表日期: 2013年1期
• 关键词: 布鲁氏菌病 PCR鉴定 原核表达 C基因 蛋白质纯化 SDS-PAGE BLOT分析 免疫原性 Brucella Wbkc gene Prokaryotic e
• 基金项目: funded by the Natural Science Foundation o China(31060352)

[Objective] In order to clone and express the formyltransferase of Brucella abortus in E.coli, purify the expressed protein and detect its immunogenicity. [Methods] A gene recoding formyltransferase 27-35 ku (Wbkc) was amplified from the genomic DNA of Br