论文部分内容阅读
采用免疫组化选择性ＤＮＡ序列分析方法，对来自河南省林州市２１例原发性食管鳞癌患者食管癌组织和癌旁基底细胞增生、间变和原位癌病灶的肿瘤抑制基因Ｐ５３进行比较研究。结果：２１例原发食管癌病灶中发现１４例Ｐ５３基因突变（６７％）。突变部位分别为第５外显子（４例，占２９％）、第６外显子（１例，占７％）、第７外显子（２例，占１４％）和第８外显子（６例，占４２％）。１例突变发生在第４内显子（７％）。对１４例癌旁上皮基底细胞增生、间变和原位癌病灶的Ｐ５３基因分析发现均有不同数量的基因突变。间变及原位癌组织的Ｐ５３基因突变与癌组织极为相似，但是基底细胞增生病变的Ｐ５３基因突变与癌组织不同，其突变部位均发生在第５外显子。提示：食管上皮不同部位的孤立病灶出现相似的分子学改变，支持食管癌多中心起源的理论，随着病变从基底细胞增生到间变到原位癌和早期浸润癌的发展，这些分子学变化可能在癌变的不同阶段起重要作用。
Immunosuppressive selective DNA sequence analysis was used to analyze the tumor suppressor gene P53 from 21 cases of primary esophageal squamous cell carcinoma in esophageal cancer tissues and adjacent basal cell proliferation, metastasis, and carcinoma in situ in Linzhou City, Henan Province. compare research. RESULTS: Fourteen p53 mutations (67%) were found in 21 cases of primary esophageal cancer lesions. The mutation sites were exon 5 (4 cases, 29%), exon 6 (1 case, 7%), exon 7 (2 cases, 14%), and 8th appearance. Children (6 cases, 42%). One mutation occurred in the fourth exon (7%). Analysis of P53 gene mutations in 14 cases of paracrine epithelial basal cell hyperplasia, anaplastic lesions, and in situ cancer lesions revealed different numbers of gene mutations. The P53 gene mutations in anaplastic and in situ cancer tissues are very similar to those in cancerous tissues. However, the P53 gene mutations in basal cell proliferation lesions are different from those in cancerous tissues, and the mutations occur in exon 5 of the tumor. Hint: Similar molecular changes occur in isolated lesions of different parts of the esophageal epithelium, supporting the theory of multicenter origin of esophageal cancer, and these molecular changes as the lesion progresses from basal cell proliferation to metastasis to the development of carcinoma in situ and early invasive carcinoma. It may play an important role in different stages of canceration.