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研究巨噬细胞极化对人前列腺癌PC-3细胞促血管形成的作用,并探讨其机制。本实验成功诱导正常男性外周血单核细胞为M0、M1、M2和TAM,发现它们在形态学上差异明显。ELISA检测各亚型巨噬细胞条件培养液(condition medium,CM)表明:TGF-β在TAM中显著高于其他亚型,IL-10高表达于M2及TAM,而IL-12在M1中高表达(P<0.05)。RT-PCR结果表明:IL-6、IL-23与CXCL9在M1中表达显著增高(P<0.05),以及miRNA let-7b、let-7c比TAM表达显著增高(P<0.01)。体外血管形成实验结果显示M2、TAM能增强PC-3细胞的促血管形成(P<0.01)。因此,TAM亚型巨噬细胞促进PC-3细胞的血管形成机制可能与miRNA let-7b、let-7c高表达以及上述细胞因子的差异性表达有关。
To investigate the effect of macrophage polarization on the proangiogenesis of human prostate cancer PC-3 cells and to explore its mechanism. The experiment successfully induced normal male peripheral blood mononuclear cells M0, M1, M2 and TAM and found that they have obvious morphological differences. The levels of TGF-β in TAM were significantly higher than those in other subtypes, while the levels of IL-10 in M2 and TAM were significantly higher than those in control (P <0.05). The results of RT-PCR showed that the expression of IL-6, IL-23 and CXCL9 in M1 was significantly increased (P <0.05), and the expression of let-7c and let-7c in miRNAs was significantly higher than that in TAM (P <0.01). The results of in vitro angiogenesis showed that M2 and TAM can enhance the angiogenesis of PC-3 cells (P <0.01). Therefore, TAM-type macrophages promote PC-3 cell angiogenesis may be related to the miRNA let-7b, let-7c high expression and the differential expression of the above cytokines.