论文部分内容阅读
目的:观察现代中药蛇床子素(osthole,OST)对多囊卵巢大鼠卵巢功能紊乱及卵巢组织过氧化物酶体增殖物激活受体γ(PPARγ)蛋白表达的调节作用。方法:36只SD大鼠随机分为模型组、OST组和对照组三组,模型组、OST组采用16周持续光照诱导雌性SD大鼠表现多囊卵巢(polycystic ovary,PCO)状态,OST组从14周开始连续3周OST腹腔注射。16周末比较各组每日阴道涂片,卵巢病理改变;称取卵巢、子宫质量,并检测血清雌二醇(estradiol,E_2)、睾酮(testosterone,T)水平及卵巢PPARγ蛋白表达水平。结果:模型组大鼠卵巢周期消失,卵巢多囊样增大,T水平升高,卵巢PPARγ蛋白表达下降;OST组卵巢发情周期增加,血清E2水平提高,卵巢增大被抑制,同时卵巢PPARγ蛋白表达水平增加,但对血清T水平无明显影响。结论:OST可能通过提高卵巢PPARγ蛋白表达水平发挥改善卵巢周期紊乱的作用,可以作为多囊卵巢综合征(polycystic ovary syndrome,PCOS)标准化中药治疗的选择。
Objective: To observe the effect of osthole (OST), a modern Chinese medicine, on the ovarian dysfunction and the expression of peroxisome proliferator - activated receptor γ (PPARγ) protein in ovarian tissues of polycystic ovary rats. Methods: Thirty-six Sprague-Dawley rats were randomly divided into three groups: model group, OST group and control group. The model group and OST group were given continuous light for 16 weeks to induce polycystic ovary (PCO) OST was injected intraperitoneally for 3 weeks starting from 14 weeks. On the 16th weekend, vaginal smear and ovarian pathological changes were compared among groups. The ovary and uterus were weighed and the levels of estradiol (E2), testosterone (T) and PPARγ protein in ovary were measured. Results: Ovarian cycle disappeared, the ovary polycystic ovary increased, the T level increased and the ovary PPARγ protein expression decreased in OST group. OST estrous cycle increased, serum E2 level increased, ovarian enlargement was inhibited, and ovary PPARγ protein The expression level increased, but no significant effect on serum T level. Conclusion: OST may play an important role in the improvement of ovarian cycle disorders by increasing the expression of PPARγ protein in ovary, which may be used as the standardization of TCM treatment for polycystic ovary syndrome (PCOS).