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目的通过设计反义寡核苷酸(ASONs)抑制激素敏感型单纯性肾病(SRSNS)呼吸道病毒基因的表达,提供激素敏感型单纯性肾病呼吸道病毒感染的证据。方法将ASONs转染至外周血单个核细胞(PBMC)后,通过RT-PCR的方法检测呼吸道病毒基因的表达。结果SRSNS活动期PBMC中呼吸道病毒基因的表达能被其相应的ASONs所抑制。呼吸道合胞病毒基因表达受抑制率为93·9%(14/15),流感病毒受抑制率为87·5%(7/8),总抑制率为91·3%(21/23)。结论呼吸道病毒确实存在于SRSNS活动期病人体内,呼吸道病毒感染作为主要触发因素,可能为SRSNS的重要发病机制。
OBJECTIVE: To provide evidence of hormone-sensitive nephrotic respiratory virus infection by designing antisense oligonucleotides (ASONs) to inhibit the expression of respiratory virus genes in steroid sensitive simple nephrotic syndrome (SRSNS). Methods ASONs were transfected into peripheral blood mononuclear cells (PBMCs), and the expression of respiratory virus genes was detected by RT-PCR. Results The expression of respiratory virus genes in PBMCs of SRSNS active stage was inhibited by their corresponding ASONs. Respiratory syncytial virus gene expression inhibition rate was 93.9% (14/15), influenza virus inhibition rate was 87.5% (7/8), the total inhibition rate was 91.3% (21/23). Conclusion Respiratory virus does exist in patients with active SRSNS. Respiratory virus infection is the main trigger and may be an important pathogenesis of SRSNS.