论文部分内容阅读
目的了解福建省II型脊髓灰质炎(脊灰)疫苗相关株病毒(VAPV2)变异特征,为证实阻断VAPV2传播提供依据。方法采用RT-PCR扩增VAPV的VP1全长和部分3D区基因序列,统计分析病毒核苷酸(nt)和氨基酸(aa)变异情况。结果 2000-2015年福建省从5 497份粪便标本中分离到219株脊灰病毒,1株为疫苗衍生脊灰病毒(VDPV),218株为VAPV,其中VAPV2占37.16%。与Sabin2株相比,47株VAPV2中VP1变异位点>5个2株(4.26%),≤2个36株(76.60%);共涉及45个位点,其中nt2909为突变热点,导致VP1的aa143位点从非极性疏水性转换成极性亲水性。13株VAPV在3D区出现重组。结论福建省大部分VAPV2变异少,VP1的aa143位点为突变热点。
Objective To understand the variation characteristics of VAPV2 in Fujian Province and provide basis for blocking the transmission of VAPV2. Methods The VP1 full length and part of 3D region of VAPV were amplified by RT-PCR. The variation of nucleotide (nt) and amino acid (aa) were analyzed statistically. Results A total of 219 poliovirus strains were isolated from 5 497 stool specimens in Fujian province during 2000-2015. One was VpVVV, and 218 was VAPV, accounting for 37.16% of the total. Compared with the Sabin2 strain, 47 strains of VAPV2 had 5 VP2 mutants (4.26%) and 2 (36.60%) less than 2 strains, involving 45 sites, of which nt2909 was a hot spot of mutation, The aa 143 site transforms from non-polar hydrophobic to polar hydrophilic. Thirteen strains of VAPV appeared to be recombinant in the 3D region. Conclusions Most VAPV2 mutations in Fujian Province are few, and the aa143 site of VP1 is a hot spot mutation.