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根据hIL－5的结构、功能特点，设计并合成了hILS＄基端19肽，用以免疫小鼠。经融合、筛选出7株抗hIL－5单克隆抗体（mAb）。利用TF－l细胞对hILS依赖生长的特性，采用Mrt法t”对mAb进行了活性测定。互材料和方法1．l动物与细胞Balb／C／J’鼠由中国科学院上海实验动物中心提供?
According to the structure and function of hIL-5, hILS $ 19-terminal peptide was designed and synthesized to immunize mice. After fusion, seven anti-hIL-5 monoclonal antibodies (mAb) were screened out. The mAb activity assay was performed using the Mrt method using the TF-1 cell-dependent growth characteristics of hILS.METHODS AND METHODS 1. Animals and Cells Balb / C / J ’mice were provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences.