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目的:观察和探讨细胞角质素CK18慢性胆囊炎患者的胆囊组织和血清中的表达及其意义。方法:35例经腹腔镜胆囊切除的慢性结石性胆囊炎患者(27例女性患者,8例男性患者,年龄在55.65±13.48岁),将患者分为两个组,A组为患慢性非活动性结石性胆囊炎者(n=10),B组为患慢性活动性胆囊炎者(n=25),在细胞凋亡早期胱门蛋白酶分裂的CK18用M30细胞凋亡酶联免疫吸附测定,总细胞角蛋白18(从凋亡及坏死细胞中分离)用M65酶联免疫吸附测定。然后计算M30/M65.结果:胱门蛋白酶分裂的CK18,特别是总CK18在胆汁中的表达远高于血清。在B组中,胱门蛋白酶分裂的CK18和总CK18的表达在胆囊组织和血清中表达差异相当大。在胆囊粘膜上皮细胞胱门蛋白酶分裂的CK18染色呈强阳性。结论:CK18在胆囊上皮细胞中表达。胱门蛋白酶分裂的CK18和总CK18在胆囊组织中的表达远高于血清中的表达。胱门蛋白酶分裂的CK18和总CK18的表达水平在活动性胆囊炎和非活动性胆囊炎中的表达并无明显差异。
Objective: To observe and explore the expression and significance of gallbladder tissue and serum in patients with cytokeratin CK18 chronic cholecystitis. Methods: Totally 35 patients with chronic cholecystitis who underwent laparoscopic cholecystectomy (27 female patients, 8 male patients, 55.65 ± 13.48 years old) were divided into two groups. Group A was chronic inactive cholecystitis (N = 10) in group B, chronic active cholecystitis in group B (n = 25), CK18 in the early stage of apoptosis as measured by M30 cell apoptosis enzyme-linked immunosorbent assay, total cell count Keratin 18 (isolated from apoptotic and necrotic cells) was assayed by M65 enzyme-linked immunosorbent assay. Then M30 / M65 was calculated.Results: CK18, especially CK18, in caspases was much higher in bile than in serum. In group B, the expression of caspases-cleaved CK18 and total CK18 in gallbladder tissue and serum differed considerably. CK18 staining of the cleaved caspases in the gallbladder mucosa epithelium was strongly positive. Conclusion: CK18 is expressed in gallbladder epithelial cells. Caspase cleavage of CK18 and CK18 in gallbladder tissue was much higher than that in serum. The expression levels of CK18 and CK18 in caspases were not significantly different between active cholecystitis and inactive cholecystitis.