论文部分内容阅读
目的探索多发性骨髓瘤（ＭＭ）患者循环血液中肿瘤前体细胞克隆及其生物学特征。方法采用细胞培养体系对ＭＭ患者外周血单个核细胞（ＰＢＭＮＣｓ）中可能存在的肿瘤前体细胞克隆进行扩增与促分化，同时用免疫细胞化学染色，形态学观察及扫描电镜技术对其生物学特性进行初步研究。结果１４例无浆细胞污染的ＭＭ患者新鲜ＰＢＭＮＣｓ样本以小淋巴样细胞为主。培养第３天时，可见较多增殖十分活跃成簇生长的免疫母细胞样Ｂ淋巴细胞，占有核细胞的３８％±１４％，胞浆Ｉｇ阴性，浆细胞相关抗原ＣＤ３８阳性，未见形态学典型的浆细胞。培养第６天时，培养物中则以典型的浆细胞团生长为主，占有核细胞的５８％±８％。这些浆细胞高表达ＣＤ３８抗原以及与原患者骨髓瘤细胞一致单克隆性胞浆Ｉｇ。结论ＭＭ患者ＰＢＭＮＣｓ中含有高增殖潜能的肿瘤前体细胞克隆，在合适的生长条件下能迅速增殖和分化为骨髓瘤细胞
Objective To explore the cloning and biological characteristics of circulating progenitor cells in patients with multiple myeloma (MM). Methods The cell culture system was used to amplify and differentiate the tumor precursor cell clones that may exist in peripheral blood mononuclear cells (PBMNCs) of MM patients. The immunocytochemical staining, morphological observation and scanning electron microscopy techniques were used to study their biology. A preliminary study of the characteristics. Results Among the 14 patients without MM-contaminated MM, fresh PBMNCs were mainly small lymphoid cells. On the 3rd day of culture, more immunocytophilic B-lymphocytes with very active growth and cluster formation were observed, occupying 38%±14% of nuclear cells, negative for cytoplasmic Ig, and positive for plasma cell-associated antigen CD38. No morphological features were observed. Plasma cells. On the sixth day of culture, typical plasma cell clusters grew mainly in the culture, accounting for 58% ± 8% of the nuclear cells. These plasma cells highly express the CD38 antigen and are consistent with the original patient’s myeloma cells in monoclonal cytoplasmic Ig. Conclusion PBMNCs from patients with MM have high proliferative potential tumor precursor cell clones and can rapidly proliferate and differentiate into myeloma cells under suitable growth conditions.