甘蔗组织培养的技术革新

• 作 者: 黄幼雄 徐耀庭 兰德安 戴光汉 吴荣来
• 机 构: 福建省龙溪农业学校作物栽培教研组,福建省龙溪农业学校作物栽培教研组,福建省龙溪农业学校作物栽培教研组,福建省龙溪农业学校作物栽培教研组,福建省龙溪农业学校作物栽培教研组,
• 出 处: 甘蔗糖业
• 发表日期: 1979年11期
• 关键词: 组织培养 愈伤组织 诱导愈伤 离体培养 移栽成活率 基本培养基 成苗 培养基成分 诱导分化 发根

用甘蔗茎锥、心叶等组织进行离体培养到形成绿色蔗苗。一般需经过诱导愈伤组织、诱导分化绿苗和绿苗长根等一次接种、两次转移培养的过程。此法存在的主要问题是:需时较长、工作量大、成本较高、发根力差和绿苗移栽成活率较低等。针对以上问题,本试验采用“MS”基本培养基附加NAA1毫克/升和IAA2毫克/升后,只需一次接种形成愈伤组织,不要再经转移培养,就可直接分化出根系旺盛的健壮蔗苗。我们将这种培养方法,叫做“一次培养成苗”法。它具有节约成本、减少

With sugar cane stem cone, heart leaves and other organizations in vitro culture to form green sugarcane seedlings. Generally need to be induced callus, induced differentiation of green seedlings and green shoots such as a long root of inoculation, two transfer culture process. The main problems with this method are: longer time, heavy workload, higher cost, poor hair rooting ability and lower transplanting rate of green seedlings. In response to the above problems, this experiment using “MS” basic medium supplemented with NAA1 mg / l and IAA2 mg / l, only a single inoculation to form callus, do not transfer the culture, you can directly differentiate strong root cane robust seedling. We will be such a training method, called “a training seedlings” method. It has the cost savings, reduced