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研究乙肝后肝硬化代偿和失代偿患者红细胞CR1分子数量及其与CR1密度相关基因多态性的关系,探讨肝炎后肝硬化红细胞膜分子的变化在肝硬化疾病发展中的意义。采用酶免疫分析间接法检测乙肝后肝硬化患者红细胞CR1分子数量;用PCR、Hind Ⅲ酶切和凝胶电泳法检测红细胞CR1密度相关基因多态性。乙肝后肝硬化患者的红细胞CR1分子数量下降(P<0.01),失代偿者尤为显著(P<0.01)。乙肝后肝硬化患者红细胞CR1分子数量下降与肝硬化发生、发展有关。红细胞CR1分子数量表达下降主要与后天性因素有关。对乙肝后肝硬化患者进行红细胞CR1分子测定或动态观察,对探讨肝炎后肝硬化的发病机理、预后和转归具有重要意义。
To study the relationship between the number of erythrocyte CR1 molecules and CR1 density-related gene polymorphism in cirrhotic patients with decompensated and decompensated hepatitis B, and to explore the significance of molecular changes of erythrocyte membrane in patients with cirrhosis after liver cirrhosis. The number of erythrocyte CR1 molecules in patients with posthepatitic cirrhosis was detected by enzyme-linked immunosorbent assay (indirect ELISA). The polymorphisms of erythrocyte CR1 density-related genes were detected by PCR, Hind Ⅲ digestion and gel electrophoresis. The number of erythrocyte CR1 molecules in patients with posthepatitic cirrhosis decreased (P <0.01), especially in decompensated patients (P <0.01). Hepatitis B cirrhosis patients with erythrocyte CR1 molecules decreased and the occurrence and development of cirrhosis. The decrease of erythrocyte CR1 expression is mainly related to acquired factors. In patients with cirrhosis of the liver hematocrit CR1 molecular determination or dynamic observation of liver cirrhosis to explore the pathogenesis, prognosis and outcome is of great significance.