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目的:合成和表征N-酚噻嗪丙磺酸,探讨其对HRP-Luminol-H2O2化学发光体系的增强作用。方法:将N-酚噻嗪丙磺酸加入HRP-Luminol-H2O2化学发光体系,记录发光强度和发光持续时间,并用于HRP含量的测定。结果:N-酚噻嗪丙磺酸可使HRP-Luminol-H2O2化学发光体系的发光强度增强,发光持续时间达30分钟以上。HRP含量在25-800 pg/mL范围内,酶含量与发光相对强度显示良好的线性关系。结论:N-酚噻嗪丙磺酸可用于酶免疫发光分析。
OBJECTIVE: To synthesize and characterize N-phenothiazine propanesulfonic acid and investigate its potentiation on HRP-Luminol-H2O2 chemiluminescence system. Methods: N-Phenothiazine propanesulfonic acid was added into HRP-Luminol-H2O2 chemiluminescence system. The luminescence intensity and luminescence duration were recorded and used to determine HRP content. Results: N-Phenothiazine propanesulfonic acid enhanced the luminescence intensity of HRP-Luminol-H2O2 chemiluminescence system, and the luminescence duration reached more than 30 minutes. HRP content in the range of 25-800 pg / mL, the enzyme content and the relative intensity of light showed a good linear relationship. Conclusion: N-Phenothiazine propanesulfonic acid can be used for enzyme immuno luminescence analysis.