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目的:掌握宁波流感监测网分离到的H1N1流感毒株耐药情况,了解其NA基因片段变异情况。方法:从宁波流感监测网中随机选取了45株H1N1流感毒株,利用RT-PCR方法扩增M2和NA基因片段并测序,用计算机软件分析结果。结果:流感毒株2005年耐烷胺类率为54.5%,2006年为100%,2007年为100%,2008年为81.8%,2009年为9%,而耐达菲率2005年-2007年都为0%,2008年为18.1%,2009年为90.9%。结论:宁波H1N1流感毒株耐烷胺类率呈先上升后下降趋势,而耐达菲率呈上升趋势。
OBJECTIVE: To master the resistance of H1N1 influenza isolates isolated from Ningbo Influenza Surveillance Network to understand the variation of NA gene fragments. Methods: Forty-five H1N1 influenza strains were randomly selected from the Ningbo Influenza Surveillance Network. The M2 and NA gene fragments were amplified by RT-PCR and sequenced. The software was used to analyze the results. Results: The influenza strains were resistant to alkanolamines in 2005 at 54.5%, 100% in 2006, 100% in 2007, 81.8% in 2008 and 9% in 2009, while the rate of Nida-Farming was between 2005 and 2007 Both were 0%, 18.1% in 2008 and 90.9% in 2009. Conclusion: The rate of resistance to alkylamines in the H1N1 influenza virus strains of Ningbo is the first to increase and then the downward trend, while the rate of Nundafin is on the rise.