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在再灌注治疗前后选择估计存活心肌、判断梗塞面积、评价疗效的可靠指标已成为人们关注的问题。抗心肌肌球蛋白单克隆抗体为人们寻找无创、有效、可靠的指标提供了一条途径。本研究旨在评估抗心肌肌球蛋白重链单抗(AMA)在动物体内的生物分布和在心肌梗塞定位显像中的作用。材料与方法 1.抗体及标记:使用本所制备的G4杂交瘤细胞株分泌的特异性单抗AMA。~(131)I-AMA用Iodogen法标记。用纸层析法测
Before and after reperfusion therapy to choose the estimated survival myocardial infarction to determine the area, to assess the reliability of indicators of efficacy has become a matter of concern. Anti-cardiac myosin monoclonal antibody provides a way for people to find non-invasive, effective and reliable indicators. This study aimed to evaluate the biodistribution of antimyocardial monoclonal antibody (McAb) in vivo and its role in the localization of myocardial infarction. MATERIALS AND METHODS 1. Antibodies and markers: AMA specific monoclonal antibodies secreted by the G4 hybridoma cell line prepared by this study were used. ~ (131) I-AMA was labeled with Iodogen method. Paper chromatography method