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神经生长因子(NGF)可促进靶神经节神经突起生长晕的生长。本文应用~3H-尿嘧啶核苷和~3H-腺胸嘧啶核苷放射自显影术进一步探讨此作用与神经元合成RNA和DNA的关系。实验材料用新生大鼠颈上节。按Maximow双盖片法进行培养。培养物分NGF组(培养液内添加NGF粗制剂)和对照组(不用CNGF),培养终止前分别转入含放射性同位素的培养液中孵育,然后取出固定、连续切片、制作自显影标本进行观察。实验结果指出:NGF组培养物被~3H-尿嘧啶核苷标记的神经元百分率及标记颗粒水平均高于对照组,而且每当培养物神经突起生长晕生长速度出现高峰的前夕,神经元~3H-尿嘧啶核苷标记颗粒水平都是明显地增高。这说明NGF可促进颈上节神经元的RNA合成,后者与神经突起的迅速生长有一定的相互关系。在~3H-胸腺嘧啶核苷标记培养物神经元的实验中,观察到NGF可促进培养第3天的颈上节少量的神经元合成DNA。
Nerve growth factor (NGF) promotes the growth of target ganglion neurite outgrowth. In this paper, ~ 3H-uridine and ~ 3H-adenosyl thymidine autoradiography to further explore the role of neuronal RNA and DNA synthesis relationship. Experimental material with neonatal rat cervical section. Maximow double-cover method for cultivation. Cultures were divided into NGF groups (medium added with NGF crude medium) and control group (without CNGF). Before culture, the cells were transferred to radioactive isotope-containing medium and incubated. Then, fixed and continuous sections were taken out to make self-developed specimens for observation . The experimental results indicated that the percentages of neurons labeled with 3H-uridine and the levels of labeled particles in the culture of NGF group were higher than those of the control group, and on the eve of the peak of halo growth in neurite outgrowth of neurons, 3H-uridine nucleus labeled particles were significantly higher levels. This shows that NGF can promote the synthesis of RNA in the superior cervical ganglion neurons, which has a certain correlation with the rapid growth of neurites. In experiments with ~ 3H-thymidine labeled cultures of neurons, it was observed that NGF promoted the formation of small amounts of neuronal synthetic DNA on the superior cervical ganglion on day 3.