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在新疆甜菜的根部和叶片分离到两种病毒分离物。回接到甜菜上均产生环斑和沿脉线纹。最后形成坏死斑。病毒粒体为20面体,平均直径28nm。除侵染甜菜外还侵染苋色藜,昆诺阿藜、番杏、菠菜等,引起局部坏死斑,在普通烟、心叶烟、菜豆、黄瓜、番茄上无症状侵染,病毒致死温度为70℃,10分钟,体外存活期13天以上,冻干病叶三年后仍有侵染力。通过PEG沉淀。差速离心,琼脂糖柱层折及蔗糖梯度离心,可得到较高浓度的纯化病毒,病毒的紫外吸收最低值在245nm,最高值在260nm。病毒外壳蛋白的分子量为54000道尔顿,病毒基因组核酸为两个组份。在琼脂双扩散实验中,能与番茄黑环病毒(TBRV)抗血清产生较弱的沉淀线,与黄瓜花叶病毒(CMV),烟草坏死病毒(TMV)。烟草环斑病毒(TRSV)不发生反应,从上述结果可以初步认为这两种分离物为番茄黑环病毒侵染甜菜的一个株系。
Two virus isolates were isolated from the roots and leaves of Xinjiang beet. Back to the beet are produced on the ring-shaped spots and veins. The final formation of necrotic spots. Virosomes are 20-sided and have an average diameter of 28 nm. In addition to the influx of sugar beet, it also invaded amaranth, quinoa, apricot, spinach and other local necrotic spots caused by asymptomatic infection on common smoke, heart leaf tobacco, kidney bean, cucumber, tomato, lethal temperature 70 ℃, 10 minutes, in vitro survival of more than 13 days, lyophilized disease leaves are still three years after the infectivity. Precipitation by PEG. Differential centrifugation, agarose column chromatography and sucrose gradient centrifugation can be higher concentrations of purified virus, the lowest UV absorption of the virus at 245nm, the highest value at 260nm. The viral coat protein has a molecular weight of 54,000 daltons and the viral genomic nucleic acid is two components. In the agar double diffusion experiment, we can produce a weak precipitation line with the tomato black ring virus (TBRV) antiserum, with cucumber mosaic virus (CMV) and tobacco virus necrosis virus (TMV). Tobacco ring spot virus (TRSV) does not react, from the above results can be initially considered that these two isolates of tomato black ring virus infected beet strains.