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基于发夹型核酸探针的高特异性识别能力以及结晶紫与单、双链DNA结合后荧光强度的差异,以发夹型核酸作为探针,构建了一种非标记型检测p53抑癌基因的荧光DNA生物传感器.此传感器不但能够区分完全互补、单碱基突变和随机目标DNA序列,而且还能识别不同突变位点的单碱基突变序列.在0.05～5.00μmol/L范围内,完全互补目标DNA的浓度与体系的荧光增强效率呈线性关系,检出限为5×10-8mol/L.该方法已应用于检测正常和单碱基突变的p53基因.
Based on the high specific recognition ability of hairpin-type nucleic acid probes and the difference of fluorescence intensity between crystal violet and single and double-stranded DNA binding, a non-labeled detection of p53 tumor suppressor gene Of the fluorescent DNA biosensor.The sensor not only can distinguish completely complementary, single base mutation and random target DNA sequence, but also can recognize single base mutation sequence of different mutation sites in the range of 0.05 ~ 5.00μmol / L, completely The concentration of complementary target DNA was linearly related to the fluorescence enhancement efficiency of the system, with a detection limit of 5 × 10-8 mol / L. This method has been applied to the detection of normal and single-base mutant p53 genes.