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用高效液相色谱同时分析饮料中多种添加剂的方法,国内外已有报道。但在分析种类、时间、分离度及灵敏度等方面仍有不足。本文采用梯度洗脱,最大吸收波长检测,基线校正等分析饮料中九种添加剂,效果良好。 实验部分 (一)仪器与试剂 Waters 209型高效液相色谱仪:μBondapak C_(18)径向加压柱,10μm,100mm×8mm i.d.。680型梯度洗脱控制器。梯度为:A.甲醇,B,0.02 mol/L乙酸铵;5％A 3min,5—20％A 2 min,20—40％A 3min,40—50％A 4 min,50—5％A 4 min。流速2ml/min。用490型可编程序多波长检测器,最大吸收波长法检测。设定的六个波长为225,230,255,258,273,600nm。满量程吸收为2.0AUFS。检测器自动基线校正。740型数据处理机绘图计算。
High performance liquid chromatography simultaneous analysis of a variety of additives in beverages at home and abroad have been reported. However, there are still some shortcomings in the analysis of types, time, resolution and sensitivity. In this paper, the use of gradient elution, maximum absorption wavelength detection, baseline correction analysis of nine additives in beverages, the effect is good. Experimental part (a) instruments and reagents Waters 209 HPLC: μ Bondapak C_ (18) radial pressure column, 10μm, 100mm × 8mm i.d .. 680 gradient elution controller. Gradient: A. methanol, B, 0.02 mol / L ammonium acetate; 5% A 3 min, 5-20% A 2 min, 20-40% A 3 min, 40-50% A 4 min, 50-5% A 4 min. Flow rate 2ml / min. With 490 programmable multi-wavelength detector, the maximum absorption wavelength detection. The six wavelengths set are 225, 230, 255, 258, 273, 600 nm. Full scale absorption is 2.0AUFS. Detector automatically baseline correction. 740 data processor drawing calculation.