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目的 探讨脂多糖(LPS)对人脑星形胶质母细胞瘤细胞(U87)10-11易位酶1(Tet1)、10-11易位酶2(Tet2)表达的影响.方法 用LPS和(或)氯胺酮刺激星形胶质细胞,Trizol法提取总RNA,检测Tet1、Tet2 mR-NA表达的变化.将细胞分为5组:对照组,不同浓度LPS组(1 mg/L、10 mg/L,分别标记为L1组、L10组),氯胺酮组(K组),LPS加氯胺酮组(L1+K组).结果 与对照组比较,LPS组Tet1 mRNA的表达显著减少,L1组尤为显著,Tet2 mRNA的表达无明显变化;而L1+K组与L1组比较Tet1 mRNA的表达明显增加.结论 LPS可以诱导人脑星形胶质母细胞瘤细胞Tet1 mRNA表达的下调,氯胺酮可以逆转这一现象.“,”Objective To investigate the effects of lipopolysaccharide(LPS)on the expression of ten -eleven translocation enzyme 1(Tet1)and ten-eleven translocation enzyme 2(Tet2)in astrocytes.Methods Astrocytes were stimulated with LPS and/or ketamine.Their total RNA was extracted by Trizol method to detect the levels of Tet 1 and Tet2 mRNA.The cells were divided into five groups:a control group,1 mg/L and 10 mg/L LPS groups(Groups L1 and L10),a ketamine group(Group K), and a LPS plus ketamine group(Group L1+K).Results Compared with the control group,the level of Tet1 mRNA in cells exposed to LPS was significantly decreased,especially Group L1.No sig-nificant difference was found in Tet 2 mRNA expression.In contrast, the amount of Tet1 mRNA was significantly in-creased in Group L1+K and Group L1.Conclusion LPS can induce the down -regulation of Tet1 mRNA expression in human astrocytoma cells which can be reversed by ketamine.