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本文建立了生物素—抗生物素蛋白酶联免疫法测定孕妇血清及尿SP_1含量。本法最低SP_1检出量为5.6μg/L,标准SP_1浓度在5.6—90μg/L范围内呈直线关系。将五个不同标准量SP_1加入正常人(非孕妇)血清中平均回收率为100±5％。标本重复测定批内批间变异系数分别为8.0％和8.5％。测定90例停经27—55天(从末次月经第一天算起)的妇女血清SP_1含量以诊断早孕,其准确率为98.89％,其中对停经40天以上早孕诊断准确率达100％。测定65例停经50天以内的妇女尿液SP_1,含量并与临床常规HCG测定方法相比较,前者诊断早孕的准确率为96.9％,而后者为93.9％。
In this paper, biotin-avidin immunosorbent assay was used to detect serum and urinary SP 1 levels in pregnant women. The minimum detectable amount of SP_1 in this method is 5.6μg / L, and the standard SP_1 concentration has a linear relationship in the range of 5.6-90μg / L. The average recoveries of five different standard SP 1 levels in normal (non-pregnant) serum were 100 ± 5%. The coefficient of variation (CV) between replicates was 8.0% and 8.5% respectively. 90 cases of menopausal 27-55 days (from the first menstruation on the first day) serum SP_1 levels to diagnose early pregnancy, the accuracy rate of 98.89%, of which more than 40 days after menopause diagnosis of early pregnancy accuracy of 100%. Determination of 65 cases of menopausal women within 50 days of urine SP_1, and compared with clinical routine HCG determination, the first diagnosis of early pregnancy accuracy was 96.9%, while the latter was 93.9%.