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目的探讨Reprimo基因3’非翻译区824位点G>C单核苷酸多态性改变与肺癌发生的关系。方法基因分型用Ta Ka Ra的Mighty Amp DNA聚合酶法,分别检测36例肺癌患者(癌症组)及其癌旁正常组织(对照组)的Reprimo基因3’非翻译区824位点G>C分布,运用χ2检验和Armitage’s趋势检验等分析其与肺癌发生的相关性。结果两组标本经Hardy-Weinberg平衡检测,对照组在Reprimo基因3’非翻译区824位点G>C基因型观测(理论)频数为34、1、1(33.06、2.88、0.06),差异有统计学意义(P=0.042),癌症组的基因型观测(理论)频数为33、1、2(33.17、4.56、0.17),差异有统计学意义(P=0.003);Reprimo基因3’非翻译区824位点G>C单核苷酸多态性与肺癌易感性的Armitage’s趋势检验差异无统计学意义(χ2=0.31,P=0.581)。结论对照组接近符合Hardy-Weinberg遗传平衡状态,而肺癌组不符合该规律;Reprimo基因3’非翻译区824位点G>C单核苷酸位点多态性可能与肺癌发生不具有相关性。
Objective To investigate the relationship between G> C single nucleotide polymorphism (SNP) at position 824 of 3’untranslated region of Reprimo gene and the occurrence of lung cancer. Methods Genotyping Mighty Amp DNA polymerase of Ta Ka Ra was used to detect the G> C (C> C) position in the 3 ’untranslated region of Reprimo gene in 36 patients with lung cancer (cancer group) and its adjacent normal tissues (control group) Distribution, the use of χ2 test and Armitage’s trend test analysis of its correlation with the occurrence of lung cancer. Results The Hardy-Weinberg equilibrium test showed that the frequencies of G> C genotype at 824 site in the 3 ’untranslated region of Reprimo gene were 34,1,1 (33.06,2.88,0.06), the difference was (P = 0.042). The frequency of genotype observation (theory) in cancer group was 33,1,2 (33.17,4.56,0.17), the difference was statistically significant (P = 0.003); 3 ’untranslated There was no significant difference in the Armitage’s trend test between the 824> G> C SNP and lung cancer susceptibility (χ2 = 0.31, P = 0.581). Conclusions The control group is close to the Hardy-Weinberg genetic balance, while the lung cancer group does not comply with this rule. The G> C single nucleotide polymorphism at the 824 site in the 3 ’untranslated region of Reprimo gene may not be associated with the occurrence of lung cancer .