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目的 :检测非小细胞肺癌 (NSCL C)组织的微血管密度 (MVD)和 P5 3蛋白表达 ,并探讨其临床意义。 方法 :取未经放、化疗的 NSCL C手术标本 71例 ,应用 F - Ag抗体和 P5 3单抗分别免疫组化检测 MVD和 P5 3。在 2 0 0倍视野下 ,每张切片计数 5个血管最丰富区的微血管密度 ,取其平均数 (a- MVD) ;在 P5 3蛋白阳性表达密集区计数 6 0 0个肿瘤细胞中的阳性表达数。结果 :a- MVD值在 P5 3蛋白阳性表达计数≥ 133组高于 P5 3蛋白阳性表达计数 <133组 ,腺癌组显著高于鳞癌组 ,并且与TNM分类和分期相关。P5 3蛋白表达于 N1～ 2 组显著高于 N0 组。结论 :NSCL C内的 MVD与组织学类型、TNM分类和分期相关 ,MVD和突变型 P5 3蛋白表达高者预后差 ,提示 MVD和突变型 P5 3可作为 NSCL C预后指标之一。
Objective: To detect the microvessel density (MVD) and P53 protein expression in non-small cell lung cancer (NSCL C) tissues and to explore its clinical significance. Methods: Seventy - one NSCL C specimens without radiotherapy and chemotherapy were used to detect MVD and P53 by F - Ag antibody and P5 3 monoclonal antibody respectively. The microvessel density of the 5 most abundant blood vessels in each slice was counted at a 200-fold magnification, and the average value (a-MVD) was taken. The positive number in the population of 600 tumor cells in which the positive expression of P53 protein was positive Number of expressions. Results: The positive expression of a-MVD in the P5 3 protein positive group ≥ 133 was higher than that in the P5 3 protein positive group (<133). The adenocarcinoma group was significantly higher than the squamous cell carcinoma group, and was associated with TNM classification and staging. P5 3 protein expression in N1 ~ 2 group was significantly higher than N0 group. CONCLUSIONS: MVD in NSCL C is correlated with histological classification, TNM classification and staging. The high expression of MVD and mutant P53 protein has poor prognosis, suggesting that MVD and mutant P53 may serve as one of the prognostic indicators of NSCL C.