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用新鲜病叶作毒源材料,比较了磷酸钾抽提——蔗糖梯密度纯化法和硼酸盐抽提——二次糖柱纯化法对TuMV的提纯效果。前者病毒含量为8.6mg/ml,后者为10.2mg/ml。用45mg病毒免疫家兔,所得抗血清的对流电泳效价为1:64,试管沉降效价为1:1024。用此抗血清对采自西安、韩城和桑树坪的白菜、甘兰和萝卜病样104份检测结果,TuMV的检出率西安为28.84％,韩城为0.96％,桑树坪为0,初步显示出TuMV流行生态学存在着地域上的差异。
The fresh diseased leaves were used as the source materials. The purification results of TuMV from potassium phosphate extraction-sucrose density gradient purification method and borate extraction-secondary sugar column purification method were compared. The former had a virus content of 8.6 mg / ml and the latter 10.2 mg / ml. Rabbits were immunized with 45mg of virus, the resulting antiserum titer 1:64, tube sedimentation titer of 1: 1024. With this antiserum, the detection results of 104 samples of cabbage, kalanchoe and radish collected from Xi’an, Hancheng and Sangshuping showed that the detection rate of TuMV was 28.84% in Xi’an, 0.96% in Hancheng, and 0 in Sangshuping There is a geographical difference in the popular TuMV ecology.