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了解新疆南疆地区犬伯氏疏螺旋体感染的情况及流行特点,本研究采用酶联免疫吸附试验法对从新疆南疆采集的160份犬血清进行抗伯氏疏螺旋体IgG抗体检测,比较不同来源及不同性别犬伯氏疏螺旋体抗体阳性率的差异,采用巢式PCR方法扩增伯氏疏螺旋体rrf(5S)~rrl(23S)rRNA间隔区片段进行血清样本的病原学检测。结果表明,160份被检血清中18份抗伯氏疏螺旋体IgG抗体阳性,阳性率为11.25%,其中,农村饲养犬的阳性率为21.81%(12/55),城市家养犬的阳性率为5.71%(6/105),差异极显著(P<0.01);雄性犬和雌性犬的抗体阳性率依次为11.63%(10/86)和10.81%(8/74),差异不显著(P>0.05)。巢式PCR检测到2份阳性样品,测序结果表明犬感染伯氏疏螺旋体属于B.garinii.基因型。
To understand the prevalence and prevalence of Borrelia burgdorferi infection in southern Xinjiang, this study tested the anti-Borrelia burgdorferi IgG antibodies against 160 Borrelia sera collected from southern Xinjiang by enzyme-linked immunosorbent assay And Borrelia burgdorferi antibody positive rate in different sexes, nested PCR method was used to amplify Borrelia burgdorferi rrf (5S) ~ rrl (23S) rRNA spacer fragment for serum sample etiological detection. The results showed that of the 160 sera tested, 18 were positive for IgG antibodies against Borrelia burgdorferi, the positive rate was 11.25%, of which, the positive rate in rural dogs was 21.81% (12/55), the positive rate in urban domestic dogs (P <0.01). The positive rates of antibody in male and female dogs were 11.63% (10/86) and 10.81% (8/74) respectively, with no significant difference (P > 0.05). Two positive samples were detected by nested PCR. Sequencing results showed that Borrelia burgdorferi belonged to B. garinii. Genotype.