论文部分内容阅读
目的了解基孔肯雅病毒SZ_20101028株的全基因组分子遗传特征。方法通过C6/36细胞从患者血清中分离得到CHIKV,针对病毒的基因组设计了6条特异性引物,对病毒的全基因组进行扩增,测序并通过生物信息学软件分析该病毒株的全基因组分子遗传特征。结果病毒SZ_20101028株的全基因组核苷酸序列长度为12 377 nt,含两个开放阅读框,编码3 722个氨基酸,两个编码区之间含有68 nt非编码连接区;发现该毒株与2010年引起东莞基孔肯雅热疫情暴发的病原体核苷酸的同源性达到了99%,进化树分析结果表明,SZ_20101028株与2010年引起东莞疫情暴发的病原体的亲缘性最高,并且和2004年引起印度洋地区疫情暴发流行的病原体同属于印度洋亚型。结论深圳市首例输入性基孔肯雅病毒属于印度洋亚型,与2010年引起东莞疫情暴发的病原体的亲缘性最高。
Objective To understand the genome-wide molecular genetic characteristics of Chikungunya virus SZ_20101028. Methods CHIKV was isolated from the serum of patients by C6 / 36 cells. Six specific primers were designed according to the genome of the virus. The whole genome of the virus was amplified, sequenced and the whole genome of the virus was analyzed by bioinformatics software Genetic characteristics. Results The full-length nucleotide sequence of the virus SZ_20101028 strain was 12 377 nt in length and contained two open reading frames encoding 3 722 amino acids with a 68 nt non-coding region between the two coding regions. The nucleotide homology of pathogens that caused Chikungunya outbreak in Dongguan in 1999 was 99%. The results of phylogenetic tree analysis showed that SZ_20101028 strain had the highest phylogenetic relationship with the pathogens that caused the outbreak in Dongguan in 2010, The pathogens causing epidemic outbreaks in the Indian Ocean belong to the Indian Ocean subtype. Conclusion The first imported chikungunya virus in Shenzhen belonged to Indian Ocean subtype, which had the highest affinity with the pathogen that caused the outbreak in Dongguan in 2010.