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目的:研究草酸铵对青霉素致大鼠癫痫模型的影响。方法:32只大鼠随机均分为4组:假手术组、模型对照组、小剂量草酸铵实验组(30 mg/kg)和大剂量草酸铵实验组(60 mg/kg),各组均记录脑电(痫样波频率、幅度及总持续时间),HE切片计数海马CA1、CA3区残存正常神经元。结果:模型对照组病理切片中大鼠海马区结构严重破坏,存活锥体细胞数明显少于假手术组(P<0.01),痫样放电总时间明显长于假手术组(P<0.01),痫样放电频率和幅度加强。大、小剂量实验组痫样放电频率和幅度均减弱,放电总时间均较模型对照组明显减少(P<0.01);两组相应脑区的存活锥体细胞数则较模型对照组明显增多(P<0.01)。结论:草酸铵能抑制癫痫大鼠的痫样放电频率、幅度和总时间,能明显抑制海马区锥体层细胞坏死,对癫痫所致的脑部损伤有缓解作用。
Objective: To study the effect of ammonium oxalate on penicillin-induced epilepsy in rats. Methods: Thirty-two rats were randomly divided into 4 groups: sham operation group, model control group, low dose ammonium oxalate test group (30 mg / kg) and high dose ammonium oxalate test group (60 mg / EEG (epileptiform wave frequency, amplitude and total duration) was recorded and HE neurons were counted in hippocampal CA1 and CA3 areas. Results: The structure of rat hippocampus in the model control group was severely damaged. The number of surviving pyramidal neurons in the model group was significantly less than that in the sham group (P <0.01), and the total epileptic discharge time was significantly longer than that in the sham group (P <0.01) Sample discharge frequency and amplitude enhancement. Compared with the model control group, the discharge frequency and amplitude of the epileptic samples in the large and small dose groups both decreased and the total discharge time was significantly decreased (P <0.01). The number of surviving pyramidal cells in the corresponding brain regions in both groups was significantly higher than that in the model control group P <0.01). CONCLUSION: Ammonium oxalate can inhibit the epileptiform discharge frequency, amplitude and total time in epileptic rats, and can significantly inhibit the pyramidal cell necrosis in the hippocampus and alleviate the brain damage induced by epilepsy.