【摘 要】
：
Targeted double-strand breaks (DSBs)in genomes can be introduced efficiently by endonucleases (Urnov et al.,2010;Jinek et al.,2012;Joung and Sander,2013),includ
【机 构】
：
Department of Anesthesiology and Surgical Intensive Care Unit,Xinhua Hospital,Shanghai Jiao Tong Uni
论文部分内容阅读
Targeted double-strand breaks (DSBs)in genomes can be introduced efficiently by endonucleases (Urnov et al.,2010;Jinek et al.,2012;Joung and Sander,2013),including zinc-finger nucleases,transcription activator-like effector nucleases,and clustered regularly inter-spaced palindromic repeats (CRISPR)/Cas9.After DSBs,DNA repair is mainly via homology-directed repair (HDR) and/or non-homologous end joining (NHEJ)(Hustedt and Durocher,2016).It was reported that genomic DNA replacement can be achieved via HDR at the site of DSBs in multiple organisms (Dickinson et al.,2013;Yang et al.,2013;Zu et al.,2013),but the efficiency is still not enough for general application,in partic-ular for replacing long DNA fragment that is more than hundreds of base pairs(bps).As NHEJ is 10-fold more active than HDR at DSB sites (Mao et al.,2008),we speculated that NHEJ can be utilized to implement long genomic DNA replacement with high efficiency.
其他文献
Bioluminescence technology has been widely used in the field of medical detection.The bioluminescent lux reporter system provides a non-invasive platform to mon
The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)outbreak be-gan in December 2019,causing the illness known as the novel coronavirus disease 2019(
SARS-CoV-2 is the virus responsible for COVID-19 disease,and since its emergence it has spread globally with the number of total cases in the tens of millions.C
Deep learning (DL) has shown explosive growth in its application to bioinformatics and has demonstrated thrillingly promising power to mine the complex relation
Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent and functional in many biological processes,by which releasing secretary factors to promo
Necrosis,a type of unwanted and passive cell demise,usually occurs under the excessive external stress and is considered to be unregulated.However,under some sp
Protein modification by small ubiquitin-like modifier(SUMO)is an important regulatory mechanism for multiple cellular pro-cesses.Although the canonical pathway
Tumour vasculature is known to be aberrant,tortuous and erratic which can have significant implications for fluid flow.Fluid dynamics in tumour tissue plays an
SARS-CoV-2 is a kind of 'smart' virus that generates complex and dynamic crosstalk with hosts.Even with thousands of publications,the researchers have only unco
环渤中地区新构造运动强烈,新近系是主要的含油层系.新近系勘探早期集中于凸起区和陡坡带,而斜坡带和凹陷区由于缺乏油气运移条件的系统性研究导致其勘探成效较差.基于前人研