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目的:了解慢性乙型肝炎患者血浆中组织因子途径抑制物(Tissue factor pathway in-hibitor,TFP)与抗凝血酶Ⅲ(Antithrombin-Ⅲ,AT-Ⅲ)的改变.方法:TFPI抗原(TFPI:Ag)采用双夹心ELISA抗原测定法,TFPI活性(TFPI:A)采用发色底物法;AT-Ⅲ抗原(AT-Ⅲ:Ag)采用免疫浊度法,AT-Ⅲ活性(AT-Ⅲ:A)采用发色底物法.结果:慢性肝炎(轻度)38例,TFPI:Ag为162.3±27.8ng/ml),TFPI:A为621.7±54.6u/ml,均高于正常值(P值各为<0.05,<0.01).ATⅢ:Ag为296.2±31.7mg/L,AT-Ⅲ:A为84.7±14.4%,均接近正常值(P>0.05).慢性肝炎(中度)10例,TFPI:Ag为162.8±28.6ng/ml,TFPI:A为666.7±55.3u/ml,均高于正常值(P值各为<0.05,<0.01).AT-Ⅲ:Ag为258.5±28.5mg/L,接近正常值(P>0.05),At-Ⅲ:A为76.6±12.1%,低于正常值(P<0.05).结论:慢性乙型肝炎无论为慢性肝炎(轻度)与慢性肝炎(中度),均有TFPI上调,表明炎症存在,而AT-Ⅲ可正常或降低,表明肝细胞分泌AT-Ⅲ有一定程度的减少.
Objective: To investigate the changes of plasma Tissue factor pathway in-hibitor (TFP) and antithrombin-Ⅲ (AT-Ⅲ) in patients with chronic hepatitis B. Methods: TFPI: (AT-Ⅲ: Ag) were measured by double sandwich ELISA. TFPI activity (TFPI: A) Results: Chronic hepatitis (mild): TFPI: Ag was 162.3 ± 27.8ng / ml, TFPI: A was 621.7 ± 54.6u / ml, all of which were higher than the normal value The value of ATⅢ: Ag was 296.2 ± 31.7mg / L, the level of AT-Ⅲ: A was 84.7 ± 14.4%, all of which were close to the normal value (P> 0.05) , TFPI: Ag was 162.8 ± 28.6ng / ml, TFPI: A was 666.7 ± 55.3u / ml, all higher than the normal value (P <0.05, / L, which was close to the normal value (P> 0.05), At-Ⅲ: A was 76.6 ± 12.1%, lower than the normal value (P0.05) .Conclusion: Chronic hepatitis B (Moderate), both TFPI up, indicating the presence of inflammation, and AT-Ⅲ can be normal or decreased, indicating that the secretion of AT-Ⅲ hepatocytes to a certain extent.